• Osahon Roli Itohan Department of Anatomy, Faculty of Basic Medical Sciences, Edo University Iyamho, Edo State, P.M.B 04, Nigeria
  • Ebeye Oladunni Abimbola Department of Human Anatomy and Cell Biology, Faculty of Basic Medical Sciences, Delta State University, Delta State, Nigeria.
  • Ehebha Santos Ehizokhale Department of Anatomy, Faculty of Basic Medical Sciences, Edo University Iyamho, Edo State, P.M.B 04, Nigeria
  • Dare Nervey Willie Department of Anatomy, Faculty of Basic Medical Sciences, Niger Delta University Wilberforce Highland, Nigeria


Background:Nandrolone Decanoate (ND) artificial testosterone in the steroid forum is of significant effect essential in clinical settings for the treatment of chronic diseases. Its mechanism of action is apparent by binding to receptor complexes such as hormones producing anabolic and androgenic effects. Objectives:To research the effects of ND on the reproductive system of Adult Wistar rats as well as on the voice of female Wistar rats exposed to the physical and non-physical activity. Methods: In this experimental study, 12 models (6 males, 6 females) received 5mg/kg BW of DecaDurabolin base on their weight indicating a normal dose once a week for four weeks. The Active models were subjected to aerobic swimming as the physical activity. Results: Female voice sounds decrease progressively from a sharp whistle-like sound to blunt and finally dull sounds. Also, the histological analysis revealed that the inactive males were with reduced spermatogenic activities, hyperplasia of the interstitial cell of Leydig with congested blood vessels in the testis while the inactive female analysis revealed foci of congestion around the cortical and medullary regions of the ovary. The histological analysis of the active males revealed reduced germ cells in the seminiferous tubules, sloughing of the germinal epithelium and interstitial edema of the testis, while the ovary revealed well-defined blood vessels within the medulla and medullary congestion. Conclusion: Administrationof Nandrolone Decanoate was associated with deleterious effects on active and inactive models on the testis, ovaries,and, voice.


Nandrolone Decanoate, Testis, Ovary, Testosterone, Wistar rat


Download data is not yet available.


Avwiworo O.G Histochemistry and tissue pathology principles and techniques. 2014 Third edition. Belardin LB, Simao VA, Leite GAA, Chuffa LGDA, Camargo ICC. (2014). Dose-dependent effects and reversibility of the injuries caused by nandrolone decanoate in uterine tissue and fertility of rats. Birth DefectsResearchPartBDevelopmentaland Reproductive Toxicology (BIRTH DEFECTS RES B)101 (2), pp168–177. BhasinS,StorerTW,BermanN,CallegariC, Clevenger B, Phillips J, Bunnell TJ, Tricker R, ShiraziA,CasaburiR.(1996)Theeffectsof supraphysiologic doses of testosterone on muscle size and strength in normal men.The New England ournal of Medicine335, pp-1–7.Bird S, Greaves RF, Burke LM, Goebel C. (2016) Doping in sport and exercise: anabolic, ergogenic, health and clinicalissues. Annals of Clinical Biochemistry. 53, pp196–221. Camargo CI, Barreiros De Souza R, Mesquita SFP, Chuffa, L, Frei, F. (2009) Ovarian histology and follicularscoreinfemaleratstreatedwith nandrolone decanoate and submitted to physical effort. Acta BiologicaHungarica60(3), pp:253–261. Camargo ICC, Leite GAA, Pinto T, Ribeiro-Paes JT. (2014) Histopathological findings in the ovaries and uterus of albino female rats promoted by co-administration of synthetic steroids and nicotine. Experimental and Toxicologic Pathology 66(4), 195–202.Creasy DM., Chapin RE., (2013) Male Reproductive System. Haschek and Rousseaux’s Handbook of Toxicologic Pathology.Ebeye OA, Ekudina VO, Osahon RI. (2016). The Histomorphologicalandphysicaleffectof Nandrolone Decanoate on the hepatorenal tissues of adult Wistarratexposed physicalactivity. European Journal of Pharmacological and Medical Research. 3(5), pp-285-289.Forbes, GB. (1985) The effect of anabolic steroids on leanbodymass:thedose-responsecurve. Metabolism. 34, pp-571–573.Frati P, Busardò FP, Cipolloni L, Dominicis ED, Fineschi V. (2015) Anabolic Androgenic Steroid (AAS) related deaths: autoptic, histopathological and toxicological findings. Current Neuropharmacology13,146–159. García-Manso JM., Esteve TV. (2016) Consequences of the use of anabolic-androgenicsteroidson female athletes’ fertility. In: Exercise and Human Reproduction. Springer.Johnson LG, Fisher G, Silvester LJ, Hofhein CC. Anabolic steroid: effect on strength, body weight, oxygen uptake and spermatogenesis upon mature males. Medicine & Science in Sports & Exercise. 1972;4(4) pp-3-5.Karbalay-Doust S, Noorafshan. A., (2012) Stereological estimation of ovarian oocyte volume, surface area andnumber:applicationonmicetreatedwith Nandrolone Decanoate. Folia HistochemCytobiol. 50(2):275-279.PanMM,KovacJR.,(2016)Beyondtestosterone cypionate: the evidence behind the use of Nandrolone in male health and wellness”. 5(2): 213-9. Rahil,J.,Saeed,S.,Ahmad,F.(2015)Effectof supraphysiological dose of Nandrolone Decanoate on the testis and testosterone concentration in mature andimmaturemalerats:Atime-coursestudy. International Journal of Reproductive Biomedicine13(12), 779-786.Saddick,S.Y.(2018)Theimpactofnandrolone decanoateadministrationonovariananduterine tissuesintherat:Luteinizinghormoneprofile, histopathological and morphometricassessment. Saudi Journal of Biological Sciences.;25(3), 507–512. Simão,V.A.,Belardin,L.B.,Leite,G.A.A.,Chuffa, L.G.A., Camargo ICC.(2015) Effects of different doses of nandrolone decanoate on estrous cycle andovarian tissue of rats after treatment and recovery periods. 96 (5): 338–349. Tamara, R.N., Vladimir, I.Z., Ivan, M.S. (2017) Acute effects of Nandrolone Decanoate on cardio dynamic parameters in isolated rat heart. Canadian Journal of Physiology and Pharmacology. 94.Tanehkar, F., Rashidy-Pour, A., Vafaei, A.A., Sameni, H.R., Haghighi, S., Miladi-Gorji, H., Bavarsad, K. (2013) Voluntary exercise does not ameliorate spatial learning and memory deficits induced by chronic administrationofnandrolonedecanoateinrats. Hormones and Behavior63(1), pp-158–165. Zirkin BR. (1998) It’s regulation by testosterone and FSH Semin cell. Developmental Biology. 9:417-21.




How to Cite

Osahon Roli Itohan, Ebeye Oladunni Abimbola, Ehebha Santos Ehizokhale, & Dare Nervey Willie. (2020). EFFECT OF NANDROLONE DECANOATE ON VOICE AND REPRODUCTIVE TISSUES IN WISTAR RAT: HISTOPATHOLOGICAL AND MORPHOMETRIC ASSESSMENT. Malaysian Journal of Medical Research (MJMR), 4(2), 1-7. Retrieved from https://ejournal.lucp.net/index.php/mjmr/article/view/995