Moringa oleifera LEAF EXTRACT LOADED HYDROGEL FOR DIABETIC WOUND HEALING
Diabetic wounds (DW) are a chronic, non-healing wound on the feet of diabetic patients that pose a serious challenge to world health. Around 84% of diabetic patients undergo lower leg amputations. Though numerous topical and systemic drugs have been used to heal the DW, these drugs have led to the emergence and subsequent rapid overgrowth of resistant bacterial strains, side effects and toxicity. Many herbal plants have very important role in wound healing because they promote the natural repair mechanisms. Moringaoleifera (MO) is an important medicinal plant which has an impressive range of medicinal uses including antimicrobial, anti-inflammatory, antidiabetic, antioxidant and anticancer activities. Recently few researchers reported that MO extracts have effective wound healing property due to the presence of rich flavonoids and vicenin-2.
The objective of the present study was to develop hydrogel formulations loaded with Moringaoleifera leaves extract. The prepared hydrogels were evaluated for physical appearance, rheological behavior, skin irritation and wound-healing power in streptozotocin-induced diabetic male wistar albino rats. Results showed that all hydrogel formulations exhibited good and acceptable physical properties. All the animals tolerated the applied gels and no signs of irritations were noticed during the skin irritation study. The in-vivowound healing studies showed a time dependent increase in percentage of wound, a contraction which is higher than that produced by the control groups. These contractions were statistically significant (P<0.001), during the first 10 days of the study with MO-Hydrogel administration. The MO-hydrogel showed the highest percent wound contraction with complete wound closure and epithelization was observed on 7thday of wound induction.
Keywords:Wound healing efficacy, Moringaoleifera, Hydrogel, Diabetic wound, Streptozotocin
Brem, H. & Tomic-Canic, M. (2007). Cellular and molecular basis of wound healing in diabetes. Journal of Clinical Investigation, 117(5), pp 1219-
Dorai, A. A. (2012). Wound care with traditional, complementary and alternative medicine. Indian Journal of Plastic Surgery, 45(2), pp 418-424.
Eldor, R., Raz, I, Ben Y , A.& Boulton, A. J. (2004). New and experimental approaches to treatment of diabetic foot ulcers: a comprehensive review of emerging treatment strategies. Diabetic Medicine, 21(11), pp 1161-1173.
Fresno Contreras,M. J., Ramírez Diéguez, A.&Jiménez Soriano,M. M.(2001). Rheological characterization of hydroalcoholic gels-15% ethanol of Carbopol Ultrez 10. Farmaco, 56(5-7), pp 437- 441.
Gothai,S.,Arulselvan,P.,Tan,W.S. & Fakurazi,S.(2016). Wound healing properties of ethyl acetate fraction of Moringa oleifera innormal human dermal fibroblasts.Journal of Intercultural Ethnopharmacology, 5(1), pp 1- 6.
Guo,J.H.(2003).Carbopol Polymers for Pharmaceutical Drug Delivery Applications, Drug development and delivery, 3(6).
Islam,M.T.,Rodríguez-Hornedo,N.,Ciotti,S.&Ackermann C.(2004).Rheological characterization of topical carbomer gels neutralized to different pH.Pharmaceutical. research,
,21(7), pp 1192-1199.
Karakkattu, V. K., Tiwari, S., Purandare, V. B.,Khedkar, S.,Bhosale,S.S. & Unnikrishnan,A.G.(2014). Choice of wound care in diabetic foot ulcer: A practical approach. World Journal of Diabetes, 5(4), pp 546- 556.
Moyer, K. E., Saggers, G. C., Allison, G. M.,Mackay, D. R.& Ehrlich, H. P. (2002). Effects of interleukin‐8 on granulation tissue maturation. Journal of cellular physiology, 193(2), pp 173-179.
National Diabetes Information Clearing house. National Diabetes Statistics fact sheet. http://diabetes.niddk.nih.gov/dm/pubs/statistics/in dex.htm.
, D.,Sahin, F.&Baris,O.(2008). Bioactivities of the various extracts and essential oils of Salvia limbata CA Mey. and Salvia sclarea L. Turkish Journal of Biology, 32(3), pp 181-192.
Patel, N. A.,Patel, M.& Patel, R. P. (2011). Formulation and evaluation of polyherbal gel for wound healing. International Research Journal of Pharmaceuticals, 1(1), pp 15-20.
Škalko, N., Bouwstra, J., Spies, F., Stuart, M., Frederik,
P.M.&Gregoriadis,G.(1998).Morphological observations on liposomes bearing covalently bound protein: Studies with freeze-fracture and cryo electron microscopy and small angle X-ray scattering techniques. Biochimica et Biophysica Acta, 1370(1), pp 151-160.
Tamburic,S. & Craig,D.Q.(1995).Rheological evaluation of polyacrylic acid hydrogels. Pharmacy and Pharmacology Communications, 1(3), pp 107-
Teoh, S. L., Latiff, A. A.& Das, S. (2009). The effect of topical extract of Momordica charantia (bitter gourd) on wound healing in nondiabetic rats and in rats with diabetes induced by streptozotocin.Clinical and experimental dermatology, 34(7), pp 815-822.
Wild, S., Roglic,G., Green,A., Sicree,R.& King, H. (2004). Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care, 27, pp 1047-1053.
Zinov'ev,E.V.,Ivakhniuk,G.K.,Dadaian,K.A.& Lagvilava,T.O.(2014).Wound-healing effect of carbopol hydrogels in rats with alloxan diabetes model.Eksperimental'naia I Klinicheskaia Farmakologiia,77(1), pp 20-25.
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