EVALUATION OF MCF-7 CELL VIABILITY BY LDH, TRYPAN BLUE AND CRYSTAL VIOLET STAINING ASSAYS
Abstract
Viability of cultured mammalian cells is evaluated by a variety of techniques. In this study, experimental results of fast cell viability assays were compared to reveal the most suitable method for determination of hyperthermia effect on viability of human breast cancer Michigan Cancer Foundation-7 (MCF-7) cell line. The cells were exposed to heat at 42˚C for 2 hours to estimate the percentage of cell viability using four assays (trypan blue, lactate dehydrogenase (LDH), and crystal violet, (CV). There was a mild decrease in percentage of cell viability as the duration of heat exposure increased. Of the three counting techniques, the crystal violet nuclei showed consistent and significantly higher value (70.58±1.97) than trypan blue and LDH assay (81.07±20.12 and 77.06±11.84 respectively) (p< 0.05). This study reveals that CV was the most sensitive assay for adherent cell. It is also very effective; simple; and permits many samples to be analyzed rapidly and simultaneously.
Keywords:
Cell viability, Crystal violet, Hyperthermia, Lactate dehydrogenase, MCF-7, Trypan blueDownloads
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