A Study on the Determination of Anti-Angiogenesis Potential of Xerochrysum Bracteatum (Everlasting Flower) Ethanolic Crude Extract by Chorioallantoic Membrane Assay of Fertilized Chick Eggs
DOI:
https://doi.org/10.31674/ijfdc.2025.v1i02.001Abstract
This research study is about the anti-angiogenesis potential of Xerochrysumbracteatum (Everlasting) flower ethanolic crude extract. Chorioallantoic membrane assay was utilized, wherein test solutions were introduced directly to the eggs’ membrane via a small opening of the egg shell.
One trial was made for the experiment, using a total of 18 chicken eggs, 6 eggs per group. The eggs were incubated for 10 days. In the experiment, the experimental group was treated with the everlasting ethanolic crude extract (EECE), positive control group - Vitamin A and the negative control group was left untreated. The eggs were re-incubated for 2 days to facilitate the absorption of the drug for the analysis. After re-incubation, the eggs were totally opened and separated to the membrane carefully to avoid blood vessels rupture. AngioTool Software was also used for quantitative assessment of various vessel morphometric and spatial parameters including vessel length and density. The data gathered was determined using One-Way ANOVA and Tukey post-hoc test. The P value which is <0.01 level is considered significant. The results showed that Everlasting Ethanolic Crude Extract (EECE) has a significant difference (number of blood vessels) and comparable effects (blood vessels length and density) with the positive control group (Retinoic Acid) for its anti-angiogenesis activity. Basing on its potency and effect, it is therefore affirmative for anti-angiogenesis.
The recommendations of this study are to conduct a study regarding the components of the Everlasting Flower that is responsible of its antiangiogenic property and also conduct a study regarding the determination of the minimal concentration for its antiangiogenic property. Most importantly, to conduct a study separating the antiangiogenic components and more.
Keywords:
Antiangiogenic, Antiangiogenic, Chorioallantoic membrane assay, Incubation, Blood vessels, Ethanolic crude extract.Downloads
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