ANTI SKIN BACTERIA ACTIVITY OF SELECTED PLANTS IN MALAYSIA
Abstract
Cosmeceuticals are topical cosmetic pharmaceutical preparations which are used to enhance the beauty through ingredients that provide additional health-related function or benefit. Natural colorants are dyes and pigmentary molecules which are extracted from nature sources such as minerals, plants and animals. A total of six plants were selected as potential candidates for this project namely Punica granatum, Dicranopteris linearis, Tagetes erecta, Hibiscus rosa sinensis (red and yellow) and Hibiscus schizopethalus. Plant samples which were freeze-dried and oven-dried were extracted using absolute methanol and subjected to rotary evaporation. For the preparation of boiled extracts, samples were boiled with distilled water for an hour prior to freeze drying. All the extracts exerted no damage to RAW 264.7 murine macrophage cell line with IC50 values more than 100 µg/ml, which was the highest concentration tested in the current study using MTT assay. A total of three species of skin bacteria namely Staphylococcus aureus (five isolates: Sa1, Sa2, Sa3, Sa4, Sa5), Staphylococcus epidermidis (five isolates: Se1, Se2, Se3, Se4, Se5) and Chryseobacterium aquifrigindense (five isolates: Ca1, Ca2, Ca3, Ca4, Ca5) were isolated from the skin swabs of thirty subjects with skin infection. From the results obtained from anti-skin bacterial testing using disc diffusion method, the extracts inhibited Staphylococcus aureus and Staphylococcus epidermidis but did not show any anti-bactericidal activity towards Chryseobacterium aquifrigindense. The highest anti-skin bacterial activity was shown by Dicranopteris linearis (oven-dried) and Punica granatum (freeze-dried and oven-dried) extracts. In conclusion, these extract have potential to inhibit the growth of skin bacteria without causing toxicity towards the skin.
Keywords:
Anti skin bacteria, Cosmeceutical, Natural colorant extractReferences
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